Composite

Part:BBa_K3852012

Designed by: YiYang Sun   Group: iGEM21_BIT-China   (2021-10-01)


T1R1+eGFP

The localization of T1R1 on the cell membrane of S. cerevisiae can be detected by attaching eGFP after T1R1.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2491
    Illegal XbaI site found at 15
    Illegal XbaI site found at 854
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1262
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2491
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2491
    Illegal BglII site found at 218
    Illegal BglII site found at 1820
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2491
    Illegal XbaI site found at 15
    Illegal XbaI site found at 854
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1262
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2491
    Illegal XbaI site found at 15
    Illegal XbaI site found at 854
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1262
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1440


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Categories
Parameters
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